Abstract
Abstract The comparative study of nick-sealing efficiency under the action of BrCN or water-soluble carbodiimide was carried out for 14 dinucleotide combinations in double helix. The difference between the lowest (17%, GpG) and the highest (94%, CpT) coupling yields was found to be more than five fold, both condensing reagents showing a similar sequence-specific trend. A strong correlation observed between coupling yields at different dinucleotide combinations and 31P NMR parameters supports the idea that variations in chemical ligation efficiency arise from sequence-specific modulations of the helix geometry and confirms close similarity of the intrinsic fine structure of intact and nicked DNAs.
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