Abstract
Previous studies have identified a sequence characterized amplified region (SCAR) marker in Salix viminalis L. that appears to segregate with gender. To characterize this marker, we tested these SCAR primers in poplar ( Populus trichocarpa Torr. & A. Gray) and 12 species of willow ( Salix ). Although amplification was inconsistent with respect to species and gender, products were obtained in four willow species (but not in poplar). The resulting sequences show that the SCAR consists of (i) a length variable purine-rich repeat region and (ii) a region highly conserved between species. The conserved region has an apparent homologue in the poplar genome, where it corresponds to the putative promoter region of an Ssu72-like gene (involved in transcriptional start site regulation) on chromosome XV. We used the poplar genome sequence to design gene-anchored primers that consistently amplify this region and part of the Ssu72-like coding region in willows as well as poplars, irrespective of species and gender. The gene-anchored primers amplify a region that, while conserved, has numerous single feature polymorphisms (SFPs) both within and between species. This region could thus be used for population and phylogenetic studies.
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