Sequence and phylogeny analysis of the complete mitochondrial ge-nome of <I>Pelteobagrus vachelli</I>

Abstract

Morphological classification of Bagridae fishes is relatively difficult due to various kinds and similar shape, and the phyletic evolution is not very clear in some species. To provide basic data to the classification of Bagridae and Siluriformes fishes, the complete mitochondrial genome of Pelteobagrus vachelli was obtained by PCR based on 16 primers, which were designed on the basis of related species mtDNA sequences. The complete mitochondrial genome is 16 527 bp in length, including 2 ribosomal RNA genes, 22 transfer RNA genes, 13 protein-coding genes, and a non-coding control region. The organization and location of genes in the mitochondrial genome of Pelteobagrus vachelli were consistent with Siluriformes fishes published in GenBank. It has high homology with other families, such as Pseudobagrus Bleeker (91%) within Siluriformes. The phylogenetic analysis of Bagridae fishes was made from the level of mitochondria genome based on 9 species of 6 genera, which belong to 4 families of the Siluriformes, combining with 3 outgroups' complete mitochondrial genomes sequences. The result indicated that Pelteobagrus vachelli, Pelteobagrus fulvidraco, Pelteobagrus nitidus and Pseudobagrus tokiensis of the Bagridae formed a monophyletic group; the Pseudobagrus Bleeker and Pelteobagrus Bleeker formed a sister group. Moreover, Pelteobagrus vachelli was more relative to Pelteobagrus nitidus than Pelteobagrus fulvidraco in Pelteobagrus Bleeker.