Abstract
Publisher Summary This chapter reviews that the determination of sequences in eukaryotic messenger RNAs has been revolutionized by the discovery of reverse transcriptase in RNA tumor viruses and as an inherent activity of DNA polymerase I of Escherichia coli. With this activity, it is possible to obtain DNA transcripts of mRNA, called complementary DNA (cDNA), by hybridization of an oligo( dT) primer to the 3'-terminal poly (A) sequence present in most eukaryotic mRNAs. This cDNA is then directly sequenced by the introduction of 32P into the transcript using α-32P-labelcd deoxyribonucleoside triphosphates. The chapter discusses that the sequences of several eukaryotic mRNAs are determined by studying the cDNA produced both with reverse transcriptase and DNA polymerase. A direct result of sequencing cDNA primed with oligo(dT) is that the RNA sequences obtained derive from the 3'-terminal regions of the mRNA, in particular from the 3' noncoding region. Therefore, it also determines the sequences of substantial sections of the 3' noncoding regions of six eukaryotic mRNAs: rabbit α- and β-globin, human α- and β-globin, mouse immunoglobulin light-chain, and chicken ovalbumin mRNAs.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Progress in Nucleic Acid Research and Molecular Biology
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
