Abstract
One viral strand of φLf, a filamentous phage ofXanthomonas campestrispv.campestris,the open reading frame (ORF440) behind gene VI was identified as gene I. This gene codes for pI protein (440 aa, 48 kDa) which was shown to be membrane-bound in the φLf-infected host cell by Western blot analysis using the antibody raised against the protein expressed inEscherichia coli.Its predicted amino acid sequence has a nucleotide-binding motif in the N-terminal 97 aa and a membrane-spanning domain (aa 221 to 236). These structural features are characteristic of pIs of several filamentous phages which are transmembrane proteins required for phage assembly. Thus far, nine φLf genes have been identified which are organized in the ordergII-gX-gV-gVII-gIX-gVIII-gIII-gVI-gI,similar to the genome organization ofE. colifilamentous phages.
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