Abstract
A full-length cDNA sequence coding for calcium-dependent protein kinase of mulberry tree, which we designated was MCDPK and this was cloned based on mulberry expressed sequence tags (ESTs). Sequence analysis showed that the MCDPK is 2249 base pairs in length, encoding 532 amino acids with a predicted molecular weight of 59.96 kDa and an isoelectric point of 6.84. The mRNA expression level ofMCDPK at different developmental stages in mulberry leaves and flowers and in different tissues was investigated. The results showed that MCDPK transcripts were most abundantly expressed in the terminal bud, and the expression level of the mRNA was reduced significantly under the conditions of low temperature and salt stress when compared to the normal growth environment. The findings of the study laid the foundation for understanding the resistance mechanism of functional genes in mulberry in the future. Key words: Mulberry, calcium-dependent protein kinase gene (CDPK), gene cloning, abiotic stress, gene expression.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
