Abstract
To convert RFLP markers of Italian ryegrass to sequence-tagged site (STS) markers, we end-sequenced 93 previously mapped single or low-copy RFLP probes. Eighty-seven clones gave acceptable results from both forward and reverse directions, and 71 contigs were detected, while other clones could not be sequenced completely because their fragments were too long. BLAST search results showed that 16 clones matched sequences reported in rice and other plants. STS primers were designed for all the 93 clones by using Oligo software, and 66 primers amplified a single band with the expected size. Of the 2 SSR primers designed from the 2 clones containing (AT)n or (TTA)n repeats, 1 amplified an SSR. Fifty-seven (85%) of the 67 STS (and SSR) primer pairs could amplify products in perennial ryegrass, 47 (70%) in meadow fescue, and 55 (82%) in tall fescue—species closely related to Italian ryegrass. Forty percent of the STS primers detected within-cultivar length or presence /absence of polymorphisms.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
