Septoria tritici Blotch Development as Affected by Temperature, Duration of Leaf Wetness, Inoculum Concentration, and Host.

Abstract

The effects of incubation temperature, leaf-wetness duration, inoculum concentration, and interaction between leaf-wetness duration and inoculum concentration on the development of Septoria tritici blotch were evaluated at the seedling stage in two bread wheats (Katepwa and 6 Lacos-78) and two durum wheats (AC Melita and Kyle). The study was conducted to assess if bread and durum cultivars widely grown in Manitoba and a resistant cultivar from South America react differently to the disease at temperatures characteristic of Manitoba summers, and to obtain information on conditions that would be used in differentiating resistant and susceptible cultivars under controlled conditions. The experiments were carried out under three temperature regimes. Factors that evaluated included inoculum concentration and duration of leaf wetness. Increasing incubation temperature, duration of leaf wetness, and inoculum concentration resulted in an increase in disease severity. There were significant (P < 0.05) differences for duration of leaf wetness and inoculum concentration within each cultivar. Pycnidia were observed 4 days earlier when incubation temperature increased from 18°C day/15°C night to 22°C day/15°C night or when inoculum concentration increased from 1 × 106 spores/ml to 1 × 107 spores/ml. There were more pycnidia when duration of leaf wetness was 72 h as opposed to 48 h and 60 h. The cultivar that was presumed to be resistant maintained its resistance under environmental conditions that are characteristic of Manitoba summers. We found that the optimal conditions for screening spring wheats for Septoria tritici blotch reaction were incubation temperatures of 18°C day/15°C night, and 22°C day/15°C night. Leaf wetness duration of 48 or 72 h and inoculum concentration of 1 × 107 spores/ml consistently produced a susceptible reaction on Katepwa, AC Melita, and Kyle the three cultivars that were susceptible to Septoria tritici blotch.