Abstract
Monoiodinated tyrosine methyl ester, a derivative of 5-hydroxy-6,8,11,14-eicosatetraenic acid containing 125I in the phenolic orhto position was prepared with high specific radioactivity and separated by column chromatography on a Sephadex LH-20 gel. The adsorption behaviour of the labelled product was studied by absorption chromatography suing a Sephadex LH-20 adsorbent with ethanol—water as a binary eluent and by reversed-phase high-performance liquid chromatograhy using C 18-silica as a stationary phase with aqueous binary containing ethanol, methanol or acetonitrile. In both separation systems, a linear relationship was found between the logarithmic capacity factor or distribution coefficient and the logarithmic concentration of the organic solvent.
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