Abstract
For the separatory determination of free glucuronic acid and conjugated glucuronic acids of various types, such as N-glucuronides and ester-type or ether-type O-glucuronides in urine, a revised method using a column of anion-exchange resin was investigated. A column of Amberlite IRA-411, after passing a mixed solution of sodium glucuronate, sodium aniline or 2-naphthylamine glucosiduronate, and phenyl glucosiduronic acid or potassium menthyl O-glucosiduronate, was first eluted with ammonium chloride solution at pH 8 to separate the free glucuronic acid, and then eluted with hydrochloric acid to hydrolyse and remove the N-glucuronide. The column was further eluted with methanolic hydrochloric acid to remove the stable O-glucuronide. For the colorimetric measurement of glucuronic acid in each of eluted fractions, a modified procedure of Dische's method using carbazole was employed, in which carbazole was added with sulfuric acid to the sample in order to save the time required for the whole procedure. By this improved method, so far as the present samples were concerned, the separatory and recovery determination of glucuronic acid, N-glucuronide, and O-glucuronide, excluding ester-type O-glucuronide, in urine, was quite satisfactory.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
