Abstract
Abstract A method for the separation and determination of biliary metabolites of equilin in rat by high-performance liquid chro-matography with electrochemical detection has been developed. After extraction of equine estrogens in bile with a C18 bonded silica cartridge, conjugated metabolites were hydrolyz-ed with sulfatase containing β-glucuronidase and free estrogens liberated were then separated on a reversed phase C18 column, the limit of detection being in the range of 5–20 pg. The transformation of equilin administered orally into 2-methoxy derivatives of equilin, equilenin and their 17β-reduced metabolites is also discussed.
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