Abstract
The separations of the immunosuppressive reagents, capsaicinoids, vitamin E, curcumins on cyclofructan based stationary phases containing polystyrene supported native cyclofructan (MCI Gel CRS100), silica supported native cyclofructan (Frulic N), silica supported isopropyl carbamate cyclofructan 6 (Larihc P), silica supported R-naphthylethyl carbamate cyclofructan 6 (Larihc RN) in normal phase HPLC mode were studied. From the investigation it showed that the column MCI Gel CRS100 was more suitable for the separation in normal phase than in hydrophilic interaction liquid chromatography. Derivatized cyclofructan stationary phases, Larihc P and Larihc RN, showed better selectivities in comparison with the native cyclofructan stationary phases. Trifluoroacetic acid had less influence on resolution on cyclofructan based stationary phases in normal phase mode.
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