Separation, quantification and identification of non-volatile organic acids in body fluids by gas chromatography

Abstract

The present paper concentrates on the analysis of non-volatile organic acids in physiological fluids. The solvent extraction and DEAE-Sephadex extraction of organic acids, respectively, seem to be the most widely used methods for isolation of this group of compounds from the biological matrix. Gas chromatography—mass spectrometry (GC/MS) is the preferred method for the separation, quantification and identification. In three tables organic acids are divided into the classes according to a functional group, a survey of alkylation methods is given and retention indices of common urinary organic acids on various GC columns are summarized.