Abstract
Urushiol, a major component of a sap of lac trees ( Rhus vernicifera), is resolved into four components due to differences in the degree of unsaturation by high-performance liquid chromatography on Hitachi 3053 gel (an 8% octadecylsilane chemically bonded silica gel), using 1% lithium perchlorate in acetonitrile-water (7:3) as eluent and electrochemical detection. This method was applied to the analysis of the urushiol components of two samples of sap and in the lac-making process.
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