Abstract
Publisher Summary This chapter discusses the separation of the subfractions of the serum triacylglycerols (TAG) by the method of thin layer chromatography (TLC). The neutral serum lipids are separated by preparative TLC on silica gel G plates using pharmaceutical benzine: diethyl ether: glacial acetic acid (85:15:2, v/v) after their extraction from the serum with chloroform: methanol (2:1, v/v). A marked separation of five fractions is obtained. They are clearly seen in day light penetrating through a glass plate. Marking with iodine vapors at the end band of the plate is used too. The TAG fraction is contoured. The TAG fraction is scraped away, extracted with diethyl ether, and a corresponding volume, depending on the TAG concentration in the serum, is laid on the plate, covering with silica gel G and impregnating with 0.5% silver nitrate in methanol. The separation is carried out in benzine fraction at 40–70°C and acetone in correlation and common quantity varying in dependence of the ambient temperature.
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