Abstract
Abstract 1. 1. Data on the separation of mitochondrial and microsomal fractions from rat liver by density-gradient electrophoresis with and without electrolyte gradients are described. Physiological cations were deliberately excluded from the system. The theoretical basis of electrophoresis in density and electrolyte gradients is discussed. 2. 2. A mitochondrial fraction prepared in the presence of EDTA is shown to consist principally of mitochondria and other particles which are probably nuclear fragments. 3. 3. Microsomes (glucose 6-phosphatase particles) have been separated from ferritin, and by means of electrophoresis on an electrolyte gradient a complex charge distribution has been demonstrated for microsomes. Other minor components are present.
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