Abstract
The separation of some water- and fat-soluble vitamins via micellar systems of reversed-phase high-performance thin-layer chromatography (HPTLC) and pressurized planar electrochromatography (PPEC) was subjected to research. Hence, the influence of the mobile phase composition (surfactant and acetonitrile concentration, eluent buffer pH) on the migration distances and zone separation of some vitamins (thiamine, riboflavin, niacin, pyridoxine, cyanocobalamin, folic acid, ergocalciferol and α-tocopherol) was investigated. Our results indicated that the applied technique has an impact on the solute order. Comparing the system capacity of HPLC and PPEC (measured as height of the theoretical plate) for the mobile phase systems with and without surfactant shows differences, especially for fat-soluble vitamin. The variances and reproducibilities (% RDS) values of the vitamin are less in PPEC than in TLC. Moreover, the migration distances of water-soluble vitamins are longer than fat-soluble ones. Overall, eluent consisting of 50% acetonitrile, 18.75 mM SDS, the buffer of pH 6.99 via the PPEC technique was most appropriate for determining the investigated vitamins in the artificial mixture and the two commercially available vitamin combinations.
Highlights
The separation of some water- and fat-soluble vitamins via micellar systems of reversed-phase highperformance thin-layer chromatography (HPTLC) and pressurized planar electrochromatography (PPEC) was subjected to research
We found the migration distance of this compound to be shorter in comparison with that of niacin
We saw that sorbent equilibration with the mobile phase before beginning the PPEC experiment improve the solute separation due to the lack of the second eluent front
Summary
The separation of some water- and fat-soluble vitamins via micellar systems of reversed-phase highperformance thin-layer chromatography (HPTLC) and pressurized planar electrochromatography (PPEC) was subjected to research. Some papers present a determination of water-soluble vitamins in natural products, e.g., rice beans[5] and wild-growing p lants[6]. In such endeavours, distinguishing of water-soluble and fat-soluble vitamins is performed separately. Considering the water-soluble vitamins of the first group, reversed-phase systems of high-performance liquid chromatography are the method of the first choice. A mixture of fat-soluble vitamins and antioxidants in biological fluids during the single-step process of RP-HPLC was separated by Lazzarino and co-workers[9]. The same method was Scientific Reports | (2021) 11:21851
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