Abstract
High-pressure cation-exchange chromatography was used to separate three of the by-product compounds formed in the chloramphenicol production process. Baseline separation can be achieved within 8.5 min employing a 1-m column packed with Zipax SCX pellicular cation exchanger and 0.05 M sodium sulphate solution as eluent at pH 1.1 in a Varian Aerograph LC 4020 liquid chromatograph.
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