Separation of Selected Bile Acids by TLC. VIII. Separation on Silica Gel 60F254 Glass Plates Impregnated with Cu(II), Ni(II), Fe(II), and Mn(II) Cations

Abstract

The aim of our study was to examine the following bile acids: cholic acid (C), glycocholic acid (GC), glycolithocholic acid (GLC), deoxycholic acid (DC), chenodeoxycholic acid (CDC), glycodeoxycholic acid (GDC), and lithocholic acid (LC). In the present study, to separate a/m bile acids using adsorption thin layer chromatography at 18°C, the glass plates precoated with silica gel 60F254 (#1.05715), were impregnated with 1%, 2.5%, and 5% aqueous solutions of the following salts: CuSO4, MnSO4, NiSO4, and FeSO4. The mixtures of n‐hexane–ethyl acetate–acetic acid in the volume compositions: 22:20:5; 25:20:2; 25:20:5, and 25:20:8 were used as mobile phases. These mobile phases were not effective for the separation of bile acids on non impregnated silica gel 60F254 plates at 18°C. The plates impregnated with the salts whose application resulted in ΔRF≥0.05 and RS>1 for all neighboring pairs of examined bile acids were considered the most effective for bile acids separation. It was observed that impregnation of silica gel 60F254 glass plates with aqueous solutions of CuSO4, MnSO4, NiSO4, and FeSO4 improved the separation of GC/GDC and C/GLC, which separated poorly on glass plates precoated with non impregnated silica gel 60F254 in adsorption TLC at 18°C.