Abstract
Immunomagnetic separation with immunomagnetic beads was used to separate Salmonella enteritidis from experimentally contaminated liquid eggs. Immunoglobulin (IgY) from egg yolk was prepared of hens immunized with S. enteritidis PT4 and its lipopolysaccharide (LPS). The specific antibodies against S. enteritidis and its LPS were produced and deposited into hen egg yolk. These antibodies were immobilized to immunomagnetic beads and used to separate S. enteritidis from liquid eggs. The antimicrobial activity of the IgY was higher when the LPS was used as an antigen rather than whole cells. The amounts of coating immunoglobulin and incubation time influenced the isolation ratio of the bacteria in the samples. Using anti-LPS antibody immobilized magnetic beads, a maximum of 93.2% of S. enteritidis was separated from the contaminated liquid eggs (102−103 colony forming units/mL). Keywords: Immunomagnetic separation; Salmonella enteritidis; egg yolk antibody; eggs; lipopolysaccharide
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