Abstract
A method is described for the separation of rapidly labeled DNA of bovine liver cells grown in vitro . The cells were lysed by sodium dodecyl sulfate and the lysate was analysed by zonal centrifugation through neutral sucrose gradients. Three rapidly labeled fractions with different sedimentation rates were obtained. The slowly sedimenting R1 possessed single-stranded properties. R2 sedimented slightly slower and R3 markedly faster than the not replicating DNA. R1 and R2 were labeled very rapidly but in R3 the label appeared after a lag time of about 5 min. It is suggested that R1 and R2 are segments of the replicating DNA molecule as conceived by Okazaki's model while R3 is related to the termination of the replication process.
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