Abstract
Eight pyridylamino (PA) derivatives of fucose-containing oligosaccharides and ten high mannose-type PA-oligosaccharides were separated by high performance liquid chromatography (HPLC) on a triacontyl (C30) silica-gel column. The eight fucose-containing PA-oligosaccharides and eight out of ten high mannose-type PA-oligosaccharides were clearly separated by C30 reversed-phase HPLC at a column temperature of 40°C with a fluorescence detector; two high mannose-type PA-oligosaccharides, Man7-GlcNAc2 and Man8-GlcNAc2, were not separated. The two unresolved PA-oligosaccharides were finally separated by C30 reversed-phase HPLC at a column temperature of 10°C. The C30 reversed-phase HPLC is quite useful for the analysis of various types of PA-oligosaccharides, including positional isomers, because of excellent separation compared with that of C18 reversed-phase HPLC. The C30 reversed-phase HPLC system can be applied to HPLC-mass spectrometry for re-confirmation of the structures of the PA-oligosaccharides.
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