Abstract
ABSTRACT Aqueous two-phase extraction using PEG (polyethylene glycol)/PK (phosphate) systems has not been used for affinity partition because of its high salt concentration, in spite of the advantage of low cost over PEG/Dex (dextran) systems. PEG modified with general dye ligands such as Procion Blue (PB) was however found to be effective for papain and some dehydrogenases in a PEG/PK system as well as in a PEG/Dex system. Separation of papain from papaya latex using PB-PEG in the PEG/PK system was successfully carried out. The inhibition constant of PB-PEG for papain was found to have nearly the same values in the PEG/PK system as in the PEG/Dex system, indicating the hydrophobic nature of the interaction. These results could be extended to some dehydrogenases suggesting that affinity partition in PEG/salt systems is a new potential method for the separation and purification of proteins.
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