Abstract
The picolinyl ester derivatives of the fatty acids of cod liver oil and of pig testis lipids were separated by high-performance liquid chromatography (HPLC) in the reversed-phase mode on a column containing an octyl-bonded phase. To obtain satisfactory resolution, it was necessary to use a mobile phase containing pyridine, which precluded the use of UV spectrophotometric detection. It was, however, possible to use the mass detector with a stream-splitter. Each of the fractions obtained was then analysed by gas—liquid chromatography—mass spectrometry, with separation on a column of fused silica coated witha a methylsilicone phase. From the mass spectra, it was possible to identify 39 and 32 different fatty acid components from cod liver oil and pig lipids respectively. A high proportion of these were not resolved when the samples were analysed without pre-fractionated by HPLC.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
