Abstract
The high-performance liquid chromatographic separation of a relative large series of simple phenolics and related substances by means of a reversed-phase system is described. The system consists of a LiChrosorb RP-18 (10 μm) Knauer column and a combination of isocratic and linear-gradient elution (solvent a, water—formic acid (95:5); solvent B, methanol; temperature, 35°C). The series of compounds studied embraces derivatives of benzoic acid (29 compounds), phenylacetic acid (11 compounds), cinnamic acid (26 compounds) and dihydrocinnamic acid (8 compounds), quinic acid esters of phenolic benzoic and cinnamic acid derivatives (9 compounds), benzaldehydes and acetophenones (25 compounds), miscellaneous phenolics (27 compounds) and coumarins (43 compounds). Amongst these substances, phenolic acids, lactones, aldehydes and ketones are represented. The possible use of the system for the analysis of complex mixtures of natural products as well as for the elucidation of the structures of more complex natural compounds is discussed.
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