Abstract
Monodimensional separation of the main DNA nucleobases, nucleosides and related 5′-nucleotides have been accomplished on dodecyltrichlorosilyl silicagel precoated thin-layer chromatography (TLC) plates using three buffered aqueous systems. This analytical procedure provides also an efficient and fast separation of the mixtures of radiation-induced derivatives of thymine and 2′-deoxyguanosine. A good correlation has been observed between the TLC migration pattern and the elution profile on octadecylsilyl silicagel high-performance liquid chromatography column for these various natural and modified DNA constituents.
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