Abstract
The effects of xenobiotics on the immune system are often diverse and can involve secondary effects on cell types other than those of primary immune origin. Thus, to better understand the effects of chemicals and drugs on immuno-competence, exposure of immune cell types to xenobiotics and assessment of functional activity have been conducted under in vitro culture conditions. Interpretation of data obtained using this approach is reliant on the known composition of the cell populations used in these assay systems as well as on the ability to obtain purified and functionally viable cells from mixed primary cell suspensions. The objective of this study was to develop an effective and reproducible means of separating murine splenic B- and T-cells into purified populations free of monocyte/macrophage and polymorphonuclear cell (PMN) contamination. Cell adherence and discontinuous Percoll gradients in conjunction with immunomagnetic negative selection or antibody-mediated complement lysis were used to obtain B...
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