Abstract
A convenient simple method for preparing sterile subpopulations of mouse spleen cells is described. Passage of mouse spleen cells through a column of Sephadex G-10 beads results in the selective retention of antibody-forming cells and of accessory cells required for in vitro induction of primary and secondary humoral immune responses to sheep red blood cells. Restoration experiments with peritoneal cells and 2-mercaptoethanol show that normal and antigen-primed T lymphocytes with helper function and B lymphocytes which are precursors of antibody-forming cells are present in substantial quantity in the effluent population. In several restoration experiments, the effects of 2-mercaptoethanol and peritoneal cells were synergistic.
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