Separation of liquid crystalline racemic mixtures obtained on the basis of (R,S)-2-hexanol on amylose tris(3-chloro-5-methylphenylcarbamate) covalently immobilised on silica in high-performance liquid chromatography

Abstract

ABSTRACT The separation on the enantiomers of thirteen newly synthesised liquid crystalline racemic mixtures obtained on the basis of (R,S)-2-hexanol was studied in HPLC on a chiral column prepared by covalent immobilisation of the chiral selector, namely amylose tris(3-chloro-5-methylphenylcarbamate) on silica. The mobile phases (MPs) consisting of n-heptane/isopropanol (IPA) or acetonitrile (ACN)/water (H2O) at different volume ratios were used. All measurements were made at room temperature. The order of the elution was also determined. The chromatographic parameters such as: resolution - (R), selectivity - (α) and number of theoretical plates - (N) were determined. On the basis of the obtained results, optimal conditions were indicated for the best separation of the tested racemic mixtures.