Abstract
Metal chelate interaction chromatography was used to separate Ig, transferrin, and albumin from blood serum and blood plasma. A column was packed with iminodiacetic acid: 1,4-butanediol diglycidyl Sepharose 6B or Sephacryl S-300 and loaded with copper, zinc, nickel, or cobalt ion. Radial immunodiffusion assay indicated that Ig-rich fractions of blood serum obtained from Zn-, Mi-, Co-, and Cu-loaded columns contained 23.2, 81.3, 79.4, and 98.1% active IgG, respectively. Transferrin was recovered from the second peak. When the same conditions of metal chelate interaction chromatography were used for blood plasma, hemoglobin tended to bind strongly to the Cu-loaded column and was eluted only with 50% ethanol. Modification of histidine residues in Ig and transferrin with diethyl pyrocarbonate almost completely destroyed their binding ability to the column. Immunoglobulin G separated showed antilipopolysaccharide antibody activity against Escherichia coli. Salmonella typhimurium, and Bordettella parapertussis.
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