Abstract
The reversed-phase retention behavior of several neutral and cationic hydrophilic thiols using trihaloacetic acid pairing agents is studied. Retention of cationic compounds increases with the size of the halogen substituent in the order: trifluoro- < trichloro- < tribromoacetic acid. The effect of pH, ionic strength, pairing ion and counter ion concentration on retention of cysteine and other thiols is measured. The formation of mobile phase ionic interactions is proposed as the mechanism of retention enhancement.
Published Version
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