Separation of Growth Promoting Factor of Broiler Chicken from Shochu Distillery By-product.

Abstract

The first experiment was conducted to show that shochu distillery by-product (SDBP) and its ether soluble fraction contains growth promoting factor of broiler chickens. The second experiment was conducted to separate the growth promoting factor of SDBP from the ether soluble fraction by column chromatography. And the third experiment was conducted to endorse the second experiment. In the first experiment, 10-day-old Cobb strain chicks were given diets containing the ether extract and its residue of SDBP. In the second experiment, 16-day-old Cobb strain chicks were given diets containing chromatographically separated fractions. The unidentified growth factor (UGF) of SDBP was separated using Sephadex LH-20 with the solvent system; water: methanol: 1, 2-dichloroethane (10:90:20). And in the third experiment, 16-day-old Cobb strain chicks were given diets containing fraction I. Its concentrations were equivalent to 0.025, 0.05 and 0.1% ether extract of SDBP. In the first experiment, it was observed that body weight gain was significantly (P<0.05) higher, and feed intake and feed conversion ratio were significantly (P<0.05) lower than the control when the diet containing 0.05% ether extract was given. However, body weight gain, feed intake and feed conversion ratio were not significantly different from the control when the diet containing 0.05% ether extract residue was given. In the second experiment, body weight gain tended to increase and feed intake and feed conversion ratio decreased when the diet containing fraction I was given. Whatever, when the diets containing fractions II, III, and IV were given, body weight gain and feed intake were significantly (P<0.05) inferior to those of the control. On the other hand, feed conversion ratio was significantly (P<0.05) higher than the control. In the third experiment, body weight gain was significantly (P<0.05) higher when the diet containing 0.1% fraction I was given. Whatever, there were not significant effects when the diets containing 0.025 and 0.05% of fraction I were given. Body weight gain and thigh muscle weight were significantly (P<0.05) higher in all the treatment groups compared with the control. Feed conversion ratios were significantly (P<0.05) lower in all the treatment groups compared with the control. These results show that the UGF occurs in ether soluble fraction, and it can be separated by column chromatography using Sephadex LH-20.