Abstract
Recent studies have demonstrated that reversed-phase high-performance liquid chromatography (RP-HPLC) is a very effective means of separating thiolate (SR)-protected gold clusters (Aun(SR)m). In the present study, we applied RP-HPLC to the separation of hydrophilic glutathionate (SG)-protected Aun(SG)m clusters. To achieve such separation, an ion pair reagent was dissolved in the solution to form ion pairs with the functional groups on the cluster surfaces, improving the interaction between the cluster surfaces and the hydrophobic stationary phase, representing ion pair chromatography. This technique resulted in the high-resolution separation of Aun(SG)m clusters via RP-HPLC. Experiments at different solution pH values led to slight improvements in the resolution, showing that pH adjustment, which modifies the efficiency of the ion pair formation process, is a useful technique for improving this type of separation.
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