Abstract
Studies on the separation of free radicals by high-performance liquid chromatography with electron spin resonance detection (HPLC-ESR) are described. Aqueous solutions of amino acids, dipeptides and tripeptides were γ-irradiated in the presence of a spin trap, 2-methyl-2-nitrosopropane. The stable aminoxyl radicals (spin adducts) produced were isolated by HPLC on cation-exchange columns. An ESR spectrometer was utilized as a detector for monitoring the eluted radicals. Various types of spin adducts were identified and characterized by ESR spectroscopy. The individual components of several pairs of diastereomeric radicals could be separated.
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