Abstract
A high-speed counter-current chromatography (HSCCC) method, using a two-phase solvent system composed of ethyl acetate/n-butanol/methanol/water (5:1:1:5, v/v/v/v), was successfully established to separate the five iridoid glucosides 7-O-ethyl sweroside (1), secologanin dimethylacetal (2), adinoside F (3), (7R)-secologain n-butyl methyl acetal (4) and adinoside G (5) from Lonicerae Japonicae Flos. Their purities were 96.8%, 98.5%, 93.3%, 98.0% and 99.9%, respectively. All the iridoid glucosides were identified by HR-ESI-MS, 1D and 2D NMR. Compounds 3 and 5 are new iridoid glucosides. The anti-inflammatory tests showed that compounds 1–5 all expressed moderate inhibitory effects on β-glucuronidase release in rat polymorphonuclear leukocytes (PMNs) induced by platelet-activating factor (PAF) with IC50 values ranging from 4.52 to 6.50 µM, while the antibacterial assays demonstrated that all the compounds displayed mild inhibitory activities against Staphylococcus aureus ATCC 25923 with MIC values ranging from 13.7 to 26.0 µg/mL.
Highlights
Lonicerae Japonicae Flos, known as Jinyinhua in the Chinese Pharmacopeia, refers to the dried flower buds or the initial flowers of Lonicera japonica Thunb
Modern phytochemistry and pharmacology studies have shown that chlorogenic acids, iridoid glucosides, polyphenols, flavonoids, triterpenoid saponins, organic acids and volatile oils were the active ingredients in Lonicera Species [3,4,5]
In the high-speed counter-current chromatography (HSCCC) separation, it is important to choose a solvent system with suitable K
Summary
Lonicerae Japonicae Flos, known as Jinyinhua in the Chinese Pharmacopeia, refers to the dried flower buds or the initial flowers of Lonicera japonica Thunb. It has been widely used in traditional. Chinese medicine to treat various diseases, such as cough, thirst, arthritis, fever, sore throat, and influenza infection [1]. It can been used in functional foods, cosmetics and other applications [2].
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