Abstract
Abstract A new C30-bonded silica column was developed for high performance liquid chromatography. This column was tested for the fractionation of fatty acids as their p-bromophenacyl esters by the reverse-phase mode. Certain pairs of fatty acid esters that are very difficult to separate on a C18-bonded silica column, i. e., arachidonic (C20:4)-palmitoleic (C16:1); elaidic (trans C18:1)-vaccinic (cis C18:1); behenic (C22:0)-nervonic (C24:1); and arachidonic (C20:0)-erucic (C22:1) esters, were completely resolved on the C30-bonded column using solvent gradients of acetonitrile: water and acetonitrile: p-dioxane. A solvent system of methylene chloride: acetonitrile (2:1, v/v) was developed for this column to achieve good separation of a homologous series of extremely nonpolar C76 to C82 α-mycolic acid esters from Mycobacterium tuberculosis H37Ra.
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