Abstract
With the extract obtained from radix peucedani by SFE(supercritical fluid extraction),coumarins were primarily purifed through silica-gel chromatography with solvent system of petroleum ether:ethyl acetate and further purification was processed by HSCCC(high speed counter current chromatography) with solvent system of petroleum ether:ethyl acetate:methanol:water(5∶5∶5∶4) at the flow rate of 2.0 mL/min under rotator speed of 850 r/min.Fractions were collected manually according to HSCCC chromatogram and then charaterized by NMR(nuclear magnetic resonance),which were peucedanocoumarin II,pteryxin,praeruptorin A and psoralen respectively.
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