Abstract
A cyclodextrin-modified micellar electrokinetic chromatography (CD-MEKC) method was developed for separating conjugated linoleic acid (CLA) isomers. All the seven CLA isomers (9 cis,11 cis-CLA, 9 cis,11 trans-CLA, 9 trans,11 trans-CLA, 10 trans,12 cis-CLA, 11 cis,13 cis-CLA, 11 cis,13 trans-CLA and 11 trans,13 trans-CLA) were completely separated in the optimized conditions (4% (w/v) β-cyclodextrin (β-CD), 54 mM sodium dodecyl sulphate (SDS), 80 mM borate (pH 9.0), 8 M urea, 4% (v/v) ethanol, 30 kV and 15 °C). The CD-MEKC method was superior to the gas chromatographic (GC) and silver-ion high-performance liquid chromatographic (Ag +-HPLC) methods that were generally used in analyzing CLA isomers.
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