Separation of complex long chain fatty acid mixtures by high-performance glass capillary gas chromatography

Abstract

Detailed descriptions are given for the preparation of high resolution open tubular glass capillary columns using SP-1000, Silar-5 CP and Silar-10 C as stationary phases. A comparison has been made between HCl etched soda lime glass capillaries and HCl leached soda lime or borosilicate glass capillaries with BaCO 3 deposition. The best results were obtained with Silar-5 CP coated on HCl leached borosilicate capillaries with BaCO 3 deposition. These types of columns have been used for the separation of complex long chain fatty acid mixtures including positional and geometrical isomers. The technique recommended has been used for 3 years and can easily be reproduced. A modification of the all-glass solvent-less injection system is also described. The qualitative and quantitative features of the glass capillary gas chromatography system are discussed.