Separation of Colloidal Particle Using Elastic-Gel-Packed Column

Hidetaka Kawakita,Shohei Esaki,Kaori Yokoyama,Keisuke Ohto,Shintaro Morisada,M Shimada

doi:10.1051/matecconf/202133304003

Abstract

Polymerized spherical gel (46 μm) was packed in a column to permeate silica particle suspension for the separation due to their differences of sizes. The elastic gel in the bottom domain of column deformed more because the pressure of the fluid flow suppressed the packed gel layer, that is, the formed gaps among the gels at the bottom domain had the smaller ‘pore’, resulting in filtering the smaller-size of the particles at the bottom of the column. The larger the size of silica particle, the upper the filtered domain in the column. To elute the filtered silica particle, the gel layer was compacted and restored by on-off of the fluid flow, to open the gaps in the gel layer repeatedly, demonstrating that the elution percentage of silica particle was gradually increased by the repeated on-off permeation of water.

Highlights

Several methods to separate various colloidal particles such as ceramics, crystal, cells and microorganisms, have been proposed
Gel deformation in a chromatographic column is typically studied under a high-speed fluid flow mathematically by Östergren and Trägrådh (1999)
A soluble monomer and initiator are dissolved in a water solution, and the solution is dropped in oil

Summary

Introduction

Several methods to separate various colloidal particles such as ceramics, crystal, cells and microorganisms, have been proposed. Colloidal particle separation using the dynamic elastic behavior of the gel layer packed in column has been proposed (Takaoka et al, 2017; Takaoka et al, 2018; Takaoka et al, 2019). Proposed elution method of the filtered particles by on-off permeation of water through elastic gel layer graphene oxide was separated by the elastic gel layer due to the difference of sizes and morphology. The elution method of the filtered colloidal particles has proposed: spherical polymerized gel was packed in the column and the suspension of silica particle was injected to the top of the packed-gel layer. Water was permeated to filter the silica particle at each domain in the gel layer. The eluted silica particle was observed and the size distribution was quantitatively determined

Materials

Preparation of spherical gel

Permeation of silica particle suspension through the gel layer

Individual permeation of silica suspension through gel layer

Recovery of filtered industrial silica particle by on-off of gel layer

Conclusions