Abstract
The aim of this work was to study and improve the separation of β-carotene cis/ trans isomers in SFC. Five different stationary phases have been tested, and their chromatographic behaviour has been determined. Unfortunately, because of the presence of additional isomers in the sample studied, none of the individual columns were able to isolate the four pure main isomers of β-carotene (9, 13, 15 cis and trans). The coupling of two different monomeric octadecyl columns was necessary to increase both selectivity and efficiency. The low viscosities of supercritical mobile phases allowed this column association. The influence of temperature, pressure, nature and modifier content were also investigated. Finally, with the optimum chromatographic conditions, six additional isomers have been separated from the four previous ones. One of them has been identified as the 9–9′ di- cis β-carotene.
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