Abstract

Foam fractionation is an effective, low-cost, and environmentally friendly method for water treatment that is widely applied to the removal of hazardous materials, organic materials, and metal ions from the wastewater by using the surfactant as a collector. This type of process known as the adsorption bubble separation technique. It uses bubbles as a separation medium and concentrates the surfactant from its aqueous solution by the difference of adsorption properties of the surfactant on gas–liquid interfaces. During the process of foam fractionation, it spared gas through the bottom of the column to create dispersed rising bubbles. They adsorbed the surfactant onto gas–liquid interfaces of the rising bubbles. We discuss here separate Bovine Serum Albumin from aqueous solution with haemoglobin by foam fraction method. To investigate the effect of the following variables on the enrichment ratio of total protein, the separation process like concentration of feed, the effect of pH, and the Effect of gas flow rate.

Highlights

  • Foam separation, which uses adsorptive bubbles to separate particles, has developed as a viable alternative to conventional separation approaches with ion exchange, chromatography, and precipitation

  • Binary protein mixtures were used in batch foam fractionation experiments to assess the enrichment ratio and percentage recovery for various feed concentrations at varying pH of the solutions, liquid pool heights, and air circulation rates

  • The effects of parameters like air flow rate, liquid pool height, feed concentration, pH of the feed, and foam height on the foam concentration and enrichment ratio were studied in experimental studies on batch foam separation of binary proteins such as Bovine serum albumin (BSA) and haemoglobin

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Summary

Introduction

Foam separation, which uses adsorptive bubbles to separate particles, has developed as a viable alternative to conventional separation approaches with ion exchange, chromatography, and precipitation. Foam fractionation is a fast, easy, and efficient method for separating chemical compounds and recovering waste products from aqueous solutions, and it can be used as a pre-concentration method in their analytical determination [1]. The authority takes the waste materials required for reuse in the food industry because of the recycling strategy of various aqueous products. The recovery of whey protein from aqueous products in the food industry is very successful when whey waste is treated as Bovine serum albumin using the foam fractionation technique in batch mode [2]. Its role of pH-induced structural change in interface-induced protein aggregation was investigated using bovine serum albumin (BSA) as a model protein to reduce protein aggregation in foam fractionation. Foam fractionation is a well-known protein purification method that may be useful in the early stages of recombinant and other proteins’ downstream production.

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