Abstract
Bacampicillin, talampicillin and pivampicillin can be separated by thin-layer chromatography on silanized silica gel with ethanol—water—2 M ammonium acetate (50:40:10) as the mobile phase. The diastereoisomers of bacampicillin and talampicillin can be separated by use of high-performance liquid chromatography. Good results are obtained with a Zorbax C 8 column (25 cm × 0.46 cm) and ethanol—water—0.2 M phosphate buffer pH 7.0 (40:55:5) as the mobile phase. The R: S ratio of bacampicillin samples varies between about 1:2 and 1:3 and for talampicillin samples R: S is always about 1:1.
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