Abstract
The physical separation of iodoamino acids has been studied in the past by means of paper and column chromatography and electrophoresis(l,2). Despite its reputation as a highly useful analytical tool in organic and biologic chemistry, few papers describing the separation of thyroid hormones by 2-dimensional TLC have appeared (3,4,5). In addition, the use of 2 solvents in the same direction to effect separations has been described (6).This communication describes a 2-dimensional method of TLC for use in the separation and quantitative recovery of radio labeled iodoamino acids and iodide and its application to analysis of rat thyroid hydrolysates.Methods and materials. Solutions of thy-roxine (T4) 3:3′: 5 triiodothyronine (T3), 3:5 diiodotyrosine (DIT), 3-monoiodotyrosine (MIT), 3:5 diiodothyronine, sodium iodide, tetraiodothyroacetic acid (TETRAC) and triiodothyroacetic acid (TRIAC) were prepared by dissolving each compound in metha-nol cone, ammonium hydroxide (99:1) so that 1 microliter of each solution ...
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Schedule a callMore From: Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)
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