Abstract
Anhydrotetracycline, epianhydrotetracycline, epitetracycline, and chlortetracycline are generally found as impurities in tetracycline. A new thin-layer partition chromatographic method is described in which the impurities are separated on a kieselguhr layer impregnated with ethylene glycol-water-acetone-ethyl acetate (2:2:15:15). Compared to previous methods, this determination is easier to perform and more versatile and it prevents rapid epimerization. The quantitative determination of the tetracyclines utilizes spectrophotometry or direct TLC fluorometry.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
