Abstract
A new high‐performance liquid chromatography technique with an evaporative light‐scattering detector (ELSD) has been developed for the separation and quantitative analysis of hydroxy and epoxy fatty acids. This method employs a gradual binary gradient (hexane/isopropanol) and ELSD detection. The minimum limit of detection is about 1 µg and ratio of mass to signal is essentially linear in the range of 10 to 200 µg. This high‐performance liquid chromatography (HPLC) technique is able to separate various positional isomers of mono‐hydroxy and dihydroxy fatty acids and can also discriminate between monohydroxy, epoxy, epoxyhydroxy, dihydroxy and trihydroxy fatty acids.
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