Abstract
Low-molecular-weight chondroitin sulfate was obtained by degradation of chondroitin sulfate using hyaluronidase. Then, separated with sephadex G25, DEAE-52, and finally purified with AKATA superpeptide separation system and fluorescence-assisted carbohydrate electrophoresis. The main compo-nents detected by high performance gel-filtration chromatography were disaccharide, tetrasaccharide, hexasaccharide with molecular weight of 521, 1024 and 1527 Da, respectively. The anti-oxidant activity of these three oligosaccharides in vitro showed that the reducing power (maximum value at 10 mg/mL) and superoxide anion radical scavenging abilities were increased (maximum value at 4 mg/mL) with an increased in their concentration. There were no significant differences of the anti-oxidant properties between those three oligosaccharides.
Highlights
The applications of high-molecular-weight polysaccharides such as glycosaminoglycans are limited by their high molecular weight, high apparent viscosity, poor water solubility, as well as complex structures and conformations
Low-molecular-weight chondroitin sulfate is a kind of acidic oligosaccharide, it can be absorbed by the solid phase in NH2-column, C18-column and silicon column very strongly, that made it very hard to elution sample
The above separation results showed that acidic oligosaccharide can be separated efficiently by combination of ion exchange and gel chromatograph
Summary
The applications of high-molecular-weight polysaccharides such as glycosaminoglycans are limited by their high molecular weight, high apparent viscosity, poor water solubility, as well as complex structures and conformations. Polysaccharides difficultly pass through organizational barriers to attach onto receptors in the cell interior (Liu et al, 2010). Regarding glycosaminoglycans, their major problem is poor bioavailability. Low-molecular-weight glycosaminoglycans are attracted increasing attention because of their biological activities. The activities of low-molecular-weight hyaluronic acid and heparin were discovered as early as in the 1990s, and extensive pertinent studies followed. Reports on the activity of chondroitin sulfate are still limited
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