Abstract
In the present work, was investigated the separation and purification procedure of the biogenic 1,3-propanediol (1,3-PD), which is a well-known valuable compound in terms of bio-based plastic materials development. The biogenic 1,3-PD was obtained as a major metabolite through the glycerol fermentation by Klebsiella pneumoniae DSMZ 2026 and was subjected to separation and purification processes. A strong acidic ion exchange resin in H+ form was used for 1,3-PD purification from the aqueous solution previously obtained by broth flocculation. The eluent volume was investigated considering the removal of the secondary metabolites such as organic acids (acetic, citric, lactic, and succinic acids) and 2,3-butanediol (2,3-BD), and unconsumed glycerol. It was observed that a volume of 84 mL of ethanol 75% loaded with a flow rate of 7 mL/min completely remove the secondary metabolites from 10 mL of concentrated fermented broth, and pure biogenic 1,3-PD was recovered in 128 mL of the eluent.
Highlights
Residual biomass, such as raw glycerol derived from biodiesel manufacturing, represents a sustainable source of nutrients for the biological catalysts that can convert it into valuable metabolites [1,2,3,4], which can further represent base materials for the production of eco-friendly plastic materials, for example, polytrimethylene-terephthalate (PTT) [5,6].Even though 1,3-PD biosynthesis from renewable biomass is very attractive from the environmental and economic point-of-view, unlike its production through chemical synthesis, the high purity of biogenic 1,3-PD needed for further industrial usages constitutes a bottleneck of the entire process [7,8]
The biogenic 1,3-PD was obtained as a major metabolite through the glycerol fermentation by Klebsiella pneumoniae DSMZ 2026 and was subjected to separation and purification processes
A strong acidic ion exchange resin in H+ form was used for 1,3-PD purification from the aqueous solution previously obtained by broth flocculation
Summary
Residual biomass, such as raw glycerol derived from biodiesel manufacturing, represents a sustainable source of nutrients for the biological catalysts that can convert it into valuable metabolites [1,2,3,4], which can further represent base materials for the production of eco-friendly plastic materials, for example, polytrimethylene-terephthalate (PTT) [5,6]. Wu et al [28] used electrodialysis through bipolar membranes to desalinate the fermentation broth, to recover the salts, and to convert them into high-added-value by-products [28] In this case, the membrane reuse limits its working life and increases the cost of the purification process. A particular aspect that makes the 1,3-PD separation process more complicated is related to its high hydrophilic characteristics, and its close boiling point to 2,3-BD and glycerol In this context, the recovery of 1,3-PD from a microbial fermentation media would consume a large amount of energy and would make up over 50% of the total production costs [9,22]. The capacity of acidic ion-exchange resin in H+ form (Amberlite IR-120H) to purify the biogenic 1,3-PD from the fermentation broth, after it was subjected to flocculation with chitosan, charcoal, and kieselguhr, was investigated
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