Abstract
The separation and indirect visible detection of inorganic and organic analyte cations on dye-coated stationary phases was studied. The dye employed in this study, Thymol Blue, is typically used for pH titrations and is composed of hydrophobic groups and a fixed charge site. The mobile phase variables that were found to affect analyte cation retention, resolution and selectivities are: concentration of dye, concentration of organic modifier, mobile phase pH, type and concentration of countercation and/or ligand, and ionic strength. Two different types of reversed-phase packings were used in this study: polymer-based stationary phases and silica-based ODS stationary phases. Detection of the analyte cations was accomplished by indirect visible detection at 428 nm.
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