Abstract
Separation and Identification of Swertiamarin from Enicostema axillare Lam.Raynal by Centrifugal Partition Chromatography and Nuclear Magnetic Resonance-Mass Spectrometry* Enicostema axillare, a bitter herb, has been used for the treatment of diabetes in traditional system of medicine in India. Swertiamarin, a bitter and potent hepatoprotective iridoid glucoside (~20 mg) was isolated by Centrifugal Partition Chromatography coupled with Diode Array Detector (DAD) from the crude methanolic extract (1.0 g) of its aerial parts using a biphasic solvent system composed of chloroformmethanol- water (86:74:20, v/v) in ascending mode for the first time. The purity of the compound was found to be >97%. The structure of swertiamarin was determined using spectroscopic data mainly NMR (1H, 13C, DEPT, HSQC and HMBC) and ESI-MS. Compound was also identified by comparing 13C NMR data with those reported and some of the 13C NMR values were revised.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
More From: Journal of Pharmaceutical Sciences & Emerging Drugs
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.